U.S. flag

An official website of the United States government, Department of Justice.

STR Data Analysis and Interpretation for Forensic Analysts

Features and Procedures: Yfiler Kit

Home  |  Glossary  |  Resources  |  Help  |  Contact Us  |  Course Map

Features have been added in GeneMapper® ID Software v3.2 to facilitate analysis of the AmpF?STR® Yfiler™ PCR Amplification Kit.

Features and Procedures (v3.2)



New feature: Allele calling parameters for new marker repeat types

In GeneMapper ID Software v3.1, allele-calling parameters were only available for markers with tetranucleotide repeat motifs. In GeneMapper® ID Software v3.2, however, allele calling parameters are available for four marker repeat types: tri-, tetra-, penta-, and hexanucleotide.

The four allele calling parameters appear in the Allele tab of the Analysis Method Editor. All related analysis values are entered directly into the Tri, Tetra, Penta, or Hexa column fields, which allows for viewing of all values.

New feature: Plus stutter filtering (for the DYS392 locus)

To aid in interpreting genotype profiles, two new fields have been added to the Allele tab of the Analysis Method Editor specifically to filter out the DYS392 plus stutter:

  • Plus Stutter Ratio
  • Plus Stutter Distance

Procedure: Workaround for the DYS19 locus (-2bp filtering)

Laboratories may choose to implement a work-around for the DYS19 locus by using the Minus A Ratio and Minus A Distance fields in the Allele tab of the Analysis Method Editor. These fields can be used to filter out the -2-bp stutter that is observed at the DYS19 locus.

Procedure: Creating HID analysis methods for the Yfiler kit

Use the Analysis Method Editor in GeneMapper® ID Software v3.2 to set analysis parameter values for analyzing the Yfiler kit data.

Procedure: Creating a table setting and uploading exported haplotype(s) for searching profiles with the Yfiler™ Haplotype Database

Using the Table Setting Editor, a table setting is created in GeneMapper® ID Sortware v3.2 to export haplotypes specifically for searching the Yfiler Haplotype Database for profile match estimation.

Genotyper® had a feature that allowed review of the sizing standard for precision assessment. This feature was not available in the first version of GMID (v3.1); it is included in version 3.2. However, it requires a work-around to achieve the precision statistics on the 250 base pair fragment. The size standards can be overlayed from all samples in the run. The base pair sizes for the fragments can be displayed, the table exported to Microsoft Excel, and the Excel functions used to calculate the 250 base pair precision from a run.

View a screen capture of the size standard overlay with the sizing table beneath:

Screen capture of the size standard overlay with the sizing table beneath
National Institute of Justice (NIJ) (see reuse policy).

Back Forward