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STR Data Analysis and Interpretation for Forensic Analysts

FMBIO® Starcall™

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STaRCall™ is the software program that genotypes gel electrophoresis data for the Hitachi FMBIO® system. Sizes from the FMBIO® Analysis Software are imported into STaRCall, similar to the process used on GS/GT software on the capillary electrophoresis platform. This program is similar in process to Genotyper®.

Allelic ladders are used to size the fragments from adjacent lanes; the closer the ladders are to the samples being run, the more precise the sizing. Where Genotyper® has a bin set associated with its macros, STaRCall™ has STR look-up tables maintained in Microsoft Excel. Optical density units are used to quantitate the fragments as the data are reviewed.

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