Archived | STR Data Analysis and Interpretation for Forensic Analysts | Genotyper®

Genotyper^® converts GeneScan^® sized peaks into genotype calls using predefined macros, providing defined results. Genotyper^® uses the tabular data from GeneScan^® to make allele calls using the first ladder recognized by the program. The two main manufacturer macros for the proprietary kits are Kazam for analysis of AmpFℓSTR^® amplification kits and PowerTyper™ for Promega products.

These macros do three things:

Calculate the bin offsets (alleles) based on the tabular data for sizing
Filter stutter as defined in the macro
Assign the number(s) that represent the genotypes for the profile (based on the ladder used for the sample set)

The macro is simply a step list of actions that are performed sequentially as defined when a particular template is launched. Macros are used to check the size standard by attaching the labeled sizes for confirmation; others can be written to label peaks and to set up Genotyper^® tables. After assignment of allele labels, the analyst proceeds with the interpretation of the overall profile.

Below is a screen shot of Genotyper®, focusing on the macro window. Click on the macro labeled "Kazam" to view the steps of the macro as they appear in text format.

Genotyper® macro window — National Institute of Justice (NIJ) (see reuse policy).

# Macro: Kazam

Find ÒladderÓ in sample info, in blue dye/lanes

Run macro ÒCalculate D3S1358 offsetsÓ

Run macro ÒCalculate vWA OffsetsÓ

Run macro ÒCalculate FGA OffsetsÓ

Find ÒladderÓ in sample info, in green dye/lanes

Run macro ÒCalculate AMEL OffsetsÓ

Run macro ÒCalculate D8S1179 offsetsÓ

Run macro ÒCalculate D21S11 OffsetsÓ

Run macro ÒCalculate D18S51 offsetsÓ

Find ÒladderÓ in sample info, in yellow dye/lanes

Run macro ÒCalculate D5S818 OffsetsÓ

Run macro ÒCalculate D13S317 OffsetsÓ

Run macro ÒCalculate D7S820 OffsetsÓ

Clear Labels

Select all categories

Unmark selected categories

Select category: D3S1358

Mark selected categories

Select blue lanes

Label category peaks with the category's name

# Following Step is Sample Allele Stutter Filter

Remove labels from peaks followed by a 809% higher, labeled peak within 3.25 to 4.75 bp

Find all ÒladderÓ in sample info, in blue dye/lanes

Label category peaks with the category's name

Remove labels from peaks whose height is less than 20% of the highest peak in a categoryÕs range

Select all categories

Unmark selected categories

Select category: vWA

Mark selected categories

Select blue lanes

Label category peaks with the category's name

# Following Step is Sample Allele Stutter Filter

Remove labels from peaks followed by a 809% higher, labeled peak within 3.25 to 4.75 bp

Find all ÒladderÓ in sample info, in blue dye/lanes

Label category peaks with the category's name

Remove labels from peaks whose height is less than 25% of the highest peak in a categoryÕs range

Select all categories

Unmark selected categories

Select category: FGA

Mark selected categories

Select blue lanes

Label category peaks with the category's name

# Following Step is Sample Allele Stutter Filter

Remove labels from peaks followed by a 809% higher, labeled peak within 3.25 to 4.75 bp

Find all ÒladderÓ in sample info, in blue dye/lanes

Label category peaks with the category's name

Remove labels from peaks whose height is less than 30% of the highest peak in a categoryÕs range

Select all categories

Unmark selected categories

Select category: AMEL

Mark selected categories

Select green lanes

Label category peaks with the category's name

# Following Step is Sample Allele Stutter Filter

Remove labels from peaks whose height is less than 3% of the highest peak in a categoryÕs range

Find all ÒladderÓ in sample info, in green dye/lanes

Label category peaks with the category's name

Remove labels from peaks whose height is less than 40% of the highest peak in a categoryÕs range

Select all categories

Unmark selected categories

Select category: D18S51

Mark selected categories

Select green lanes

Label category peaks with the category's name

# Following Step is Sample Allele Stutter Filter

Remove labels from peaks followed by a 525% higher, labeled peak within 3.25 to 4.75 bp

Find all ÒladderÓ in sample info, in green dye/lanes

Label category peaks with the category's name

Remove labels from peaks whose height is less than 10% of the highest peak in a categoryÕs range; then remove labels from peaks followed by a 222% higher, labeled peak within 0.00 to 5.00 bp

Select all categories

Unmark selected categories

Select category: D21S11

Mark selected categories

Select green lanes

Label category peaks with the category's name

# Following Step is Sample Allele Stutter Filter

Remove labels from peaks followed by a 669% higher, labeled peak within 3.25 to 4.75 bp

Find all ÒladderÓ in sample info, in green dye/lanes

Label category peaks with the category's name

Remove labels from peaks whose height is less than 20% of the highest peak in a categoryÕs range

Select all categories

Unmark selected categories

Select category: D8S1179

Mark selected categories

Select green lanes

Label category peaks with the category's name

# Following Step is Sample Allele Stutter Filter

Remove labels from peaks followed by a 733% higher, labeled peak within 3.25 to 4.75 bp

Find all ÒladderÓ in sample info, in green dye/lanes

Label category peaks with the category's name

Remove labels from peaks whose height is less than 30% of the highest peak in a categoryÕs range

Select all categories

Unmark selected categories

Select category: D5S818

Mark selected categories

Select yellow lanes

Label category peaks with the category's name

# Following Step is Sample Allele Stutter Filter

Remove labels from peaks followed by a 900% higher, labeled peak within 3.25 to 4.75 bp

Find all ÒladderÓ in sample info, in yellow dye/lanes

Label category peaks with the category's name

Remove labels from peaks whose height is less than 25% of the highest peak in a categoryÕs range

Select all categories

Unmark selected categories

Select category: D13S317

Mark selected categories

Select yellow lanes

Label category peaks with the category's name

# Following Step is Sample Allele Stutter Filter

Remove labels from peaks followed by a 900% higher, labeled peak within 3.25 to 4.75 bp

Find all ÒladderÓ in sample info, in yellow dye/lanes

Label category peaks with the category's name

Remove labels from peaks whose height is less than 25% of the highest peak in a categoryÕs range

Select all categories

Unmark selected categories

Select category: D7S820

Mark selected categories

Select yellow lanes

Label category peaks with the category's name

# Following Step is Sample Allele Stutter Filter

Remove labels from peaks followed by a 1011% higher, labeled peak within 3.25 to 4.75 bp

Find all ÒladderÓ in sample info, in yellow dye/lanes

Label category peaks with the category's name

Remove labels from peaks whose height is less than 25% of the highest peak in a categoryÕs range

Select all categories

Unmark selected categories

Select categories: D3S1358, vWA, FGA, AMEL, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820

Mark selected categories

Sort categories by: (1) ascending dye color, (2) ascending size or scan

Sort dye/lanes by: (1) ascending lane number, (2) ascending dye color

Select blue lanes

And select green lanes

And select yellow lanes

Unmark selected dye/lanes

Find ÒladderÓ in sample info, in blue dye/lanes

Mark selected dye/lanes

Find ÒladderÓ in sample info, in green dye/lanes

Mark selected dye/lanes

Find ÒladderÓ in sample info, in yellow dye/lanes

Mark selected dye/lanes

Select blue lanes

Show the plot window

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Date Created: July 10, 2023

STR Data Analysis and Interpretation for Forensic Analysts

Genotyper®

Macro Kazam in Text Format

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