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Amplified DNA Product Separation for Forensic Analysts


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Amplified DNA Product Separation for Forensic Analysts

Amplified DNA Product Separation
National Institute of Justice (NIJ) (see reuse policy).

This course provides information in three lessons.

Amplification and Electrophoresis Overview. Learn the process of electrophoresis to identify the components for successful separation of amplified DNA products, the differences between the methods of agarose gel and polyacrylamide electrophoresis, and the methods used to detect amplified DNA products separated using different types of slab-gel electrophoresis apparatus.

Capillary Electrophoresis. Learn the basic principles of capillary electrophoresis, advantages of capillary electrophoresis over slab gels, capillary electrophoresis system components, how capillary electrophoresis systems function, the instruments used by the forensic science community, and how to troubleshoot ABI Prism® instruments.

Sample Preparation and Handling. Learn the denaturation process of amplified DNA products for sample injection, the importance of formamide quality, the quality control measures for assessing formamide quality, and the pros and cons of using water in place of formamide.


Taking the Course

Starting the course. Select the top link in the navigation on the right.

Navigating the course. We recommend you go through the course page-by-page using the Forward and Back buttons at the bottom of the page. You may skip around and select lessons using the navigation on the right.


Information addressed in this interactive training program is delivered in two modules. Select a module below to begin.



Capillary Electrophoresis

Sample Preparation and Handling