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STR Data Analysis and Interpretation for Forensic Analysts

FMBIO Analysis Software

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FMBIO® Analysis Software
National Institute of Justice (NIJ) (see reuse policy).

FMBIO® Analysis Software is a program that is associated with slab gel electrophoresis. This program uses the Macintosh operating system.

Before using the FMBIO® Analysis Software, the analyst must use the read image program to define the scan area and resolution. It is similar to operating a desktop scanner. Each image captured by the read image program is converted to a Tag Image File Format (TIFF) file.01

Following scanning, the FMBIO® Analysis Software is utilized to size the bands and quantitate peak height and area. This functionality is equivalent to the GeneScan® process discussed previously. The FMBIO® Analysis Software also uses algorithms to size and quantitate. The software includes a band-finding program to aid in the identification of possible DNA fragments, but user review is required.

The following figure depicts the FMBIO® procedure, including the applicable software processes:01

FMBIO® procedure
National Institute of Justice (NIJ) (see reuse policy).

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