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DNA Amplification for Forensic Analysts

Values for Tm

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An important consideration in primer design is to ensure that the 3' and 5' primers do not have very different values for Tm. Primers with a greater than 5°C difference in Tm may result in inadvertent preferential amplification of the most efficiently primed product strand. Large differences in Tm can lead to reduction in yield or even no amplification.

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