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The distance between the 5' ends of the two primers defines the length of the amplicon. In general, primers distanced < 2000 bases apart are used. This allows for sufficient amplification of the target region.
The amplicon length is calculated as:
Product length = (Position of antisense primer - Position of sense primer) +1
Note: |
|---|
| The "+1" represents the non-template +A addition by the Taq polymerase |
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Date Created: July 31, 2023