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DNA Amplification for Forensic Analysts

No Long Runs With The Same Base

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Primers with long runs of a single base (more than three) should generally be avoided as it can cause breathing of the primer and aid in mispriming. For example, the sequence AGCGGGGGATGGGT has run of five for the base G.

Schematic of Stutter Product Formation Process
National Institute of Justice (see reuse policy).

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