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Primers have a theoretical optimal annealing temperature based on their sequence. Each primer intended for inclusion in the multiplex may have a different optimal annealing temperature. Therefore, it is important to select primers that are known to be robust and amplify successfully at less than optimal temperatures. Optimized multiplexes containing well-designed primers provide high yields of amplified product.
Additional Online Courses
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- Laboratory Orientation and Testing of Body Fluids and Tissues
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Date Created: August 25, 2023