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DNA Amplification for Forensic Analysts


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National Institute of Justice (see reuse policy).

Designing successful multiplexes can be challenging. The process begins by deciding which STR loci are suitable to incorporate into a multiplex.

Factors in deciding the loci include:

  • Locus size and overlap
  • Number of alleles
  • Thermal cycling parameters
  • Primers
  • Dyes

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