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DNA Amplification for Forensic Analysts

Applied Biosystems

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Although other kits were available, Applied Biosystem's AmpFℓSTR® and Promega's PowerPlex® kits were the main kits used during the CODIS STR Standardization Project.

The CODIS STR Standardization Project identified thirteen core loci, together with amelogenin: 

The CODIS STR Standardization Project identified thirteen core loci, together with amelogenin
National Institute of Justice (see reuse policy).

The two manufacturers responded rapidly by developing new kits that included the thirteen core loci and amelogenin. The manufacturers involved the forensic science community in the beta testing of the kits, requested feedback, and responded to numerous suggested changes. The cooperation was mutually beneficial. The industry was able to develop technology that would be readily accepted by the community; the community enabled industry to build a robust technology tailored to forensic STR testing.

Applied Biosystems retooled AmpFℓSTR® Profiler™ and developed AmpFℓSTR® Profiler Plus™ and AmpFℓSTR® COfiler™.

Applied Biosystems Profiler Plus
National Institute of Justice (see reuse policy).
Applied Biosystems COfiler
National Institute of Justice (see reuse policy).
Applied Biosystems Profiler PlusApplied Biosystems COfiler

The loci included in these Biosystems are: (highlighted loci are included in both kits)

Comparison of Applied Biosystems Kits

AmpFℓSTR® Profiler Plus™AmpFℓSTR® COfiler™
D3S1358D3S1358
D5S818 
D7S820D7S820
D8S1179D16S539
D13S317CSF1PO
D18S51TH01
FGATPOX
vWA 
AmelogeninAmelogenin

The combination of these two kits allows laboratories to test all core loci; the overlapping loci serve as a quality control measure in the testing process.

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