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Although other kits were available, Applied Biosystem's AmpFℓSTR® and Promega's PowerPlex® kits were the main kits used during the CODIS STR Standardization Project.
The CODIS STR Standardization Project identified thirteen core loci, together with amelogenin:
The two manufacturers responded rapidly by developing new kits that included the thirteen core loci and amelogenin. The manufacturers involved the forensic science community in the beta testing of the kits, requested feedback, and responded to numerous suggested changes. The cooperation was mutually beneficial. The industry was able to develop technology that would be readily accepted by the community; the community enabled industry to build a robust technology tailored to forensic STR testing.
Applied Biosystems retooled AmpFℓSTR® Profiler™ and developed AmpFℓSTR® Profiler Plus™ and AmpFℓSTR® COfiler™.
Applied Biosystems Profiler Plus | Applied Biosystems COfiler |
The loci included in these Biosystems are: (highlighted loci are included in both kits)
Comparison of Applied Biosystems Kits | |
---|---|
AmpFℓSTR® Profiler Plus™ | AmpFℓSTR® COfiler™ |
D3S1358 | D3S1358 |
D5S818 | |
D7S820 | D7S820 |
D8S1179 | D16S539 |
D13S317 | CSF1PO |
D18S51 | TH01 |
FGA | TPOX |
vWA | |
Amelogenin | Amelogenin |
The combination of these two kits allows laboratories to test all core loci; the overlapping loci serve as a quality control measure in the testing process.
Additional Online Courses
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- DNA – A Prosecutor’s Practice Notebook
- Crime Scene and DNA Basics
- Laboratory Safety Programs
- DNA Amplification
- Population Genetics and Statistics
- Non-STR DNA Markers: SNPs, Y-STRs, LCN and mtDNA
- Firearms Examiner Training
- Forensic DNA Education for Law Enforcement Decisionmakers
- What Every Investigator and Evidence Technician Should Know About DNA Evidence
- Principles of Forensic DNA for Officers of the Court
- Law 101: Legal Guide for the Forensic Expert
- Laboratory Orientation and Testing of Body Fluids and Tissues
- DNA Extraction and Quantitation
- STR Data Analysis and Interpretation
- Communication Skills, Report Writing, and Courtroom Testimony
- Español for Law Enforcement
- Amplified DNA Product Separation for Forensic Analysts