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Positive controls are included in most commercial DNA analysis kits used for quantitation or STR typing. A commonly used positive amplification control is 9947A which is a female cell line from a 31 year old Caucasian female. Some labs use other amplification controls including 007, 9948 or 2800M. Whichever one is used, it is usually amplified with each batch of samples and aids in determining the overall performance of the amplification and typing procedures.
The positive control should show the expected alleles. If no peaks are seen, this may indicate a problem with the amplification or injection. In the event that the positive control does not yield the correct results, analysts should troubleshoot the problem and follow the procedures established by the laboratory.
The positive control should show the expected alleles. If no peaks are seen, this may indicate a problem with the amplification or injection. In the event that the positive control does not yield the correct results, analysts should troubleshoot the problem and follow the procedures established by the laboratory.
Note: |
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It is expected that injection issues would have been identified in the internal size standard evaluation step. |
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