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DNA Extraction and Quantitation for Forensic Analysts


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Upon successful completion of this unit of instruction, the student shall be able to:

  • List the reasons quantitation is necessary
  • List the advantages and disadvantages of the principal DNA quantitation methods
  • Understand the basic underlying chemistry involved in several quantitation methods including slot blots, luminescent reactions, and quantitative PCR
  • Discuss proprietary kits available for DNA quantitation commonly used in forensic laboratories
  • Perform calculations and dilutions to target specific amounts of DNA suitable for PCR

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