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Resources by Module
- DNA Analysis Considerations
- Organic Extraction
- Chelex 100 Extraction
- Other Extraction Techniques
- Quantitation
DNA Analysis Considerations
Works Cited
- Wilson, I. G. 1997. Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63 (10): 3741–51.
- Harry, M., B. Gambier, Y. Bourezgui, and E. Garnier-Sillam. 1999. Evaluation of purification procedures for DNA extracted from organic rich samples: Interference with humic substances. Analusis 27 (5): 439–42.
- Al-Soud, W. A., and P. Radstrom. 2001. Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 39 (2): 485–93.
- Shutler, G. G., Gagnon, P., Verret, G., Kalyn, H., Korkosh, S., Johnston, E., and Halverson, J. 1999. Removal of a PCR inhibitor and resolution of DNA STR types in mixed human-canine stains from a five year old case. J Forensic Sci 44 (3): 623–6.
- Innis, Michael A., David H. Gelfand, John J. Sninsky, and Thomas J. White, eds. 1990. PCR protocols: A guide to methods and applications. San Diego, CA: Academic Press.
- Bourke, M. T., C. A. Scherczinger, C. Ladd, and H. C. Lee. 1999. NaOH treatment to neutralize inhibitors of Taq polymerase. J Forensic Sci 44 (5): 1046–50.
- Chung, D. T., J. Drabek, K. L. Opel, J. M. Butler, B. R. McCord. 2004. A study on the effects of degradation and template concentration on the amplification efficiency of the STR Miniplex primer sets. J Forensic Sci 49 (4): 733–40.
Works Cited
- Lorente, M., C. Entrala, J. A. Lorente, J. C. Alvarez, E. Villanueva, B. Budowle. 1998. Dandruff as a potential source of DNA in forensic casework. J Forensic Sci 43 (4): 901–2.
- Drobnic, K. 2003. Analysis of DNA evidence recovered from epithelial cells in penile swabs. Croatian Medical Journal 44 (3): 350–54.
- Schmerer, W. M., S. Hummel, and B. Herrmann. 1999. Optimized DNA extraction to improve reproducibility of short tandem repeat genotyping with highly degraded DNA as target. Electrophoresis 20 (8): 1712–16.
- Primorac, D., S. Andelinovic, M. Definis-Gojanovic, I. Drmic, B. Rezic, M. M. Baden, M. A. Kennedy, M. S. Schanfield, S. B. Skakel, and H. C. Lee. 1996. Identification of war victims from mass graves in Croatia, Bosnia, and Herzegovina by use of standard forensic methods and DNA testing. J Forensic Sci 41(5): 891–94.
- Zehner, R., J. Amendt, and R. Krettek. 2004. STR typing of human DNA from fly larvae fed on decomposing bodies. J Forensic Sci 49(2): 337–40.
- Baechtel, F. S. 1989. The extraction, purification and quantification of DNA. In Proceedings of the International Symposium on the Forensic Aspects of DNA Analysis, 25–28. Washington, D.C.: U.S. Government Printing Office.
- Comey, C. T. 1994. DNA extraction strategies for amplified fragment length polymorphism analysis. J Forensic Sci 39 (5): 1254–69.
- Yoshida, K., K. Sekiguchi, N. Mizuno, K. Kasai, I. Sakai, H. Sato, and S. Seta. 1995. The modified method of two-step differential extraction of sperm and vaginal epithelial cell DNA from vaginal fluid mixed with semen. Forensic Sci Int 72(1): 25–33.
Online Links
- Differex™ System web page
http://www.promega.com/catalog/catalogproducts.asp?catalog_name= Promega_Products&category_name=Differex+System&cookie_test=1 - Millipore Corporation Website
UltraClean™ page on MO BIO's website
http://www.mobio.com/products/categorylist.php?cat_id=5
Chelex 100 Extraction
Works Cited
- Walsh, P. S., D. A. Metzger, and R. Higuchi. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10 (4): 506–13.
- Suenaga, E., and H. Nakamura. 2005. Evaluation of three methods for effective extraction of DNA from human hair. J Chromatography Biology Analyt Technol Biomed Life Sci 820 (1): 137–41.
- Vandenberg N., R. A. van Oorschot, and R. J. Mitchell. 1997. An evaluation of selected DNA extraction strategies for short tandem repeat typing. Electrophoresis 18 (9): 1624–6.
- Tsuchimochi, T., M. Iwasa, Y. Maeno, H. Koyama, H. Inoue, I. Isobe, R. Matoba, M. Yokoi, and M. Nagao. 2002. Chelating resin-based extraction of DNA from dental pulp and sex determination from incinerated teeth with Y-chromosomal alphoid repeat and short tandem repeats. Am J Forensic Med Pathol 23 (3): 268–71.
- Sweet, D., M. Lorente, A. Valenzuela, J. A. Lorente, and J. C. Alvarez. 1996. Increasing DNA extraction yield from saliva stains with a modified Chelex method. Forensic Sci Int 83 (3): 167–77.
- Greenspoon, S. A., M. A. Scarpetta, M. L. Drayton, and S. A. Turek. 1998. QIAamp spin columns as a method of DNA isolation for forensic casework. J Forensic Sci 43 (5): 1024–30.
- Hoff-Olsen, P., B. Mevag, E. Staalstrom, B. Hovde, T. Egeland, and B. Olaisen B. 1999. Extraction of DNA from decomposed human tissue: An evaluation of five extraction methods for short tandem repeat typing. Forensic Sci Int 105 (3): 171–83.
- Comey, C. T. 1994. DNA extraction strategies for amplified fragment length polymorphism analysis. J Forensic Sci 39 (5): 1254–69.
- Yoshida, K., K. Sekiguchi, N. Mizuno, K. Kasai, I. Sakai, H. Sato, and S. Seta. 1995. The modified method of two-step differential extraction of sperm and vaginal epithelial cell DNA from vaginal fluid mixed with semen. Forensic Sci Int 72 (1): 25–33.
Other Extraction Techniques
Works Cited
- Corning Life Sciences. 2006. Product catalog entry, Costar® Spin-X® centrifuge tube insert without membrane, nonsterile, 1000/case (item #9301).
- Millipore Corporation. 2006. Product catalog entry, Microcon® centrifugal filter units. http://www.millipore.com/catalogue.nsf/docs/C3034
- Horsman, K. M., S. L. Barker, J. P. Ferrance, K. A. Forrest, K. A. Koen, and J. P. Landers. 2005. Separation of sperm and epithelial cells in a microfabricated device: Potential application to forensic analysis of sexual assault evidence. Analytical Chemistry 77 (3): 742–49.
- Greenspoon, S. A., M. A. Scarpetta, M. L. Drayton, and S. A. Turek. 1998 . QIAamp spin columns as a method of DNA isolation for forensic casework. J Forensic Sci 43 (5): 1024–30.
- Sinclair, K., and V. M. McKechnie. 2000. DNA extraction from stamps and envelope flaps using QIAamp and QIAshredder. J Forensic Sci 45 (1): 229–30.
- Vandenberg, N., and R. A. van Oorschot. 2002. Extraction of human nuclear DNA from feces samples using the QIAamp DNA Stool Mini Kit. J Forensic Sci 47 (5): 993–95.
- Greenspoon, S. A., J. D. Ban, K. Sykes, E. J. Ballard, S. S. Edler, M. Baisden, and B. L. Covington. 2004. Application of the BioMek 2000 Laboratory Automation Workstation and the DNA IQ System to the extraction of forensic casework samples. J Forensic Sci 49 (1): 29–39.
- Nagy, M., P. Otremba, C. Kruger, S. Bergner-Greiner, P. Anders, B. Henske, M. Prinz, and L. Roewer. 2005. Optimization and validation of a fully automated silica-coated magnetic beads purification technology in forensics. Forensic Sci Int 152 (1): 13–22.
- Salvadore, J. M., and M. C. De Ungria. 2003. Isolation of DNA from saliva of betel quid chewers using treated cards. J Forensic Sci 48 (4): 794–97.
- Thacker C., C. P. Phillips, and D. Syndercombe-Court. 2000. Use of FTA cards in small volume PCR reactions. Progress in Forensic Genetics 8 (1999): 473–75.
Online Links
- Filter Paper Cards on Whatman's website
http://www.whatman.com/products/?pageID=7.31 - UltraClean™ page on MO BIO's website
http://www.mobio.com/products/categorylist.php?cat_id=5
Quantitation
Works Cited
- Baechtel, F. S. 1989. The extraction, purification and quantification of DNA. In Proceedings of the International Symposium on the Forensic Aspects of DNA Analysis, 25–28. Washington, D.C.: U.S. Government Printing Office.
- Turner BioSystems. YEAR? Fluorescence theory. In An introduction to fluorescence measurements. (accessed August 31, 2006).
- Turner BioSystems. A TD-700 Laboratory Fluorometer Method for PicoGreenR" Turner Designs. Luminometers and Fluorometers
- "QuantiBlotTM. A DNA Quantitation Method" Perkin Elmer, April 1996. 7-15
- Walsh, P. S., J. Varlaro, and R. Reynolds. 1992. A rapid chemiluminescent method for quantitation of human DNA. Nucleic Acids Res 20 (19): 5061-65.
- Promega Corporation. 2006. Technical Bulletin, AluQuant® Human DNA Quantitation System, instructions for use of products DC1010 and DC1011, part #TB291. (accessed August 31, 2006)
- Edwards, Kirstin, Julie Logan, and Nick Saunders, eds. 2004. Real-time PCR: An essential guide, 4–5. Wymondham, Norfolk, U.K.: Horizon Bioscience.
- Edwards, Kirstin, Julie Logan, and Nick Saunders, eds. 2004. Real-time PCR: An essential guide, 18–22. Wymondham, Norfolk, U.K.: Horizon Bioscience.
- Edwards, Kirstin, Julie Logan, and Nick Saunders, eds. 2004. Real-time PCR: An essential guide, 45–46. Wymondham, Norfolk, U.K.: Horizon Bioscience.
- Timken, Mark D., Katie L. Swango, Cristián Orrego, Mavis Date Chong, and Martin R. Buoncristiani. 2005. Quantitation of DNA for forensic DNA typing by qPCR (quantitative PCR): Singleplex and multiplex modes for nuclear and mitochondrial genomes, and the Y chromosome. Final report for U.S. Department of Justice grant no. 2002-IJ-CX-K008.
- Department of Biology, Davidson College. 2001. Real-time PCR method. Genomics course materials http://www.bio.davidson.edu/courses/ genomics/method/realtimepcr.html (accessed August 31, 2006).
- Edwards, Kirstin, Julie Logan, and Nick Saunders, eds. 2004. Real-time PCR: An essential guide, 51–52. Wymondham, Norfolk, U.K.: Horizon Bioscience.
- Applied Biosystems. 2003. QuantifilerTM Human DNA Quantification Kit and QuantifilerTM Y Human DNA Quantification Kit User's Manual, rev. B.
- Butler, John M. 2005. Forensic DNA typing: Biology, technology, and genetics of STR markers. 2nd ed, 77–78. Burlington, MA: Elsevier Academic Press.
Online Links
- Abstract for AluQuant® Human DNA Quantitation System
http://www.promega.com/applications/hmnid/productprofiles/aluquant/Default.htm - FRET at Wikipedia
http://en.wikipedia.org/wiki/Fluorescence_resonance_energy_transfer
Additional Online Courses
- What Every First Responding Officer Should Know About DNA Evidence
- Collecting DNA Evidence at Property Crime Scenes
- DNA – A Prosecutor’s Practice Notebook
- Crime Scene and DNA Basics
- Laboratory Safety Programs
- DNA Amplification
- Population Genetics and Statistics
- Non-STR DNA Markers: SNPs, Y-STRs, LCN and mtDNA
- Firearms Examiner Training
- Forensic DNA Education for Law Enforcement Decisionmakers
- What Every Investigator and Evidence Technician Should Know About DNA Evidence
- Principles of Forensic DNA for Officers of the Court
- Law 101: Legal Guide for the Forensic Expert
- Laboratory Orientation and Testing of Body Fluids and Tissues
- DNA Extraction and Quantitation
- STR Data Analysis and Interpretation
- Communication Skills, Report Writing, and Courtroom Testimony
- Español for Law Enforcement
- Amplified DNA Product Separation for Forensic Analysts
Date Created: July 6, 2023