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Historically, DNA extraction was accomplished by mechanical or chemical disruption of cells to release their organelles and contents. This works well in samples containing many cells, but has required adaptation for use with the much smaller biological samples collected at crime scenes.
Organic extraction is a conventional method that uses organic chemicals to isolate genomic DNA. The procedure can be described in four steps:
- solubilization of the stain components
- denaturation and hydrolysis of proteins
- removal of denatured proteins
- purification of DNA06
Additional Online Courses
- What Every First Responding Officer Should Know About DNA Evidence
- Collecting DNA Evidence at Property Crime Scenes
- DNA – A Prosecutor’s Practice Notebook
- Crime Scene and DNA Basics
- Laboratory Safety Programs
- DNA Amplification
- Population Genetics and Statistics
- Non-STR DNA Markers: SNPs, Y-STRs, LCN and mtDNA
- Firearms Examiner Training
- Forensic DNA Education for Law Enforcement Decisionmakers
- What Every Investigator and Evidence Technician Should Know About DNA Evidence
- Principles of Forensic DNA for Officers of the Court
- Law 101: Legal Guide for the Forensic Expert
- Laboratory Orientation and Testing of Body Fluids and Tissues
- DNA Extraction and Quantitation
- STR Data Analysis and Interpretation
- Communication Skills, Report Writing, and Courtroom Testimony
- Español for Law Enforcement
- Amplified DNA Product Separation for Forensic Analysts