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Many extraction procedures incorporate the use of a microcentrifuge tube with a spin basket. Costar Spin-X® centrifuge tube filters are one example. These centrifuge tube inserts contain no membrane, but have a bonded fritted bottom on the 500µL insert that fits into the microcentrifuge tube.1,2
This allows for the separation of liquid carrying the biological material from the substrate during centrifugation steps. Another modification is the use of centrifugal filter units. These allow for extracted DNA to be purified and concentrated after extraction and prior to amplification. Millipore Microcons® are one example of centrifugal filter units.2 Both of these modifications are routinely used in DNA extraction procedures.
Additional Online Courses
- What Every First Responding Officer Should Know About DNA Evidence
- Collecting DNA Evidence at Property Crime Scenes
- DNA – A Prosecutor’s Practice Notebook
- Crime Scene and DNA Basics
- Laboratory Safety Programs
- DNA Amplification
- Population Genetics and Statistics
- Non-STR DNA Markers: SNPs, Y-STRs, LCN and mtDNA
- Firearms Examiner Training
- Forensic DNA Education for Law Enforcement Decisionmakers
- What Every Investigator and Evidence Technician Should Know About DNA Evidence
- Principles of Forensic DNA for Officers of the Court
- Law 101: Legal Guide for the Forensic Expert
- Laboratory Orientation and Testing of Body Fluids and Tissues
- DNA Extraction and Quantitation
- STR Data Analysis and Interpretation
- Communication Skills, Report Writing, and Courtroom Testimony
- Español for Law Enforcement
- Amplified DNA Product Separation for Forensic Analysts