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Crime Scene and DNA Basics for Forensic Analysts


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Image of AmpFLP (D1S80) visualized using silver staining
AmpFLP (D1S80) visualized using silver staining
National Institute of Justice (NIJ) (see reuse policy).

The next phase in the development of DNA for use in forensic science returned to the concept of VNTRs. Some VNTR regions are relatively short and can be amplified by PCR.

Sometimes called AmpFLPs (amplified fragment poymorphisms), examples that were evaluated for use in crime laboratories included:

  • D1S80
  • YNZ 22 (D17S5)
  • 3'ApoB

AmpFLPs did not have the discrimination power of RFLP or the simplicity of DQ-Alpha/Polymarker and were not widely used.

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