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Crime Scene and DNA Basics for Forensic Analysts

Objectives

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Upon successful completion of this unit of instruction, the student shall be able to do the following:

  • Understand the history of forensic science and the use of restriction fragment length polymorphisms (RFLP)
  • Understand the method in which DNA profiles are developed, including the use of oligonucleotides as probes
  • Understand the disadvantages of RFLP analysis
  • Understand the concept of short tandem repeats (STR) testing, including multiplexing and the ability to label nucleotides with fluorescent tags
  • Describe the history of forensic DNA testing
  • Describe the advantages of STR typing over earlier methods
  • Describe the sequence of steps involved in DNA typing

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