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The importance of avoiding cross contamination cannot be overemphasized. The investigator performing the collection must ensure tools are clean or sterilized and that gloves are changed between handling each sample.
Collection methods differ depending on the type of evidence and the substrate upon which it is found. It is preferable to collect evidence in its original state. If the evidence is fragile or can easily be lost, the entire object should be collected and packaged, if size and circumstances permit.
Some laboratories recommend the submission of substrate controls. Substrate controls are clean samples of the collection materials or unstained portions of the material the biological evidence is deposited on. The laboratories can use these to troubleshoot contamination, Polymerase Chain Reaction (PCR) inhibition, or interference with fluorescence.
The investigator should consult the local forensic laboratory and refer to the department standard operating procedures regarding collection and preservation of biological evidence.
Procedures for Evidence Collection |
|
Blood & Other Body Fluids |
|
Type of Collection |
Procedure |
Cuttings |
Removal of a section of the item containing the stain using a sterile or clean cutting device. |
Wet Absorption |
A sterile swab, gauze pad, or threads are slightly moistened with sterile distilled water. An effort should be made to concentrate the stain in a localized portion of the swab or pad. For example, when using a swab, the stain should be concentrated on the tip. The collection medium is concentrated into the stain and allowed to air dry. Some laboratories recommend following the first moistened swabbing with a second dry swabbing to ensure thorough sample collection. Both swabs are retained and submitted for analysis. |
Scraping Method |
Using a clean razor blade or scalpel, the sample is scraped into a clean piece of paper that can be folded and packaged in a paper envelope or other appropriate packaging. |
Lifting with Tape |
For dried blood stains on a non-absorbent surface, fingerprint lifting tape may be placed over the stain and lifted off. The stain is transferred to the adhesive side of the tape, which may then be secured on a clear piece of acetate for submission to the laboratory. |
Hair & Fiber Collection |
|
Type of Collection |
Procedure |
Visual Collection |
On some surfaces, hairs and fibers can be seen with the naked eye. Using clean forceps and trace paper, the sample can be removed from the surface and placed into a clean piece of paper that can be folded and packaged in a paper envelope or other appropriate packaging. |
Tape Lifting |
Water or methanol soluble tapes are available for the collection of trace hair and fiber evidence. The tape is applied to the location of the suspected sample, removed, and packaged. |
Vacuuming Method |
The area where the suspected samples are located are vacuumed up and caught in a filtered trap attached to the vacuum. These samples are packaged in clean trace paper for submission to the laboratory. Vacuuming is the least desirable collection method because there is a risk of cross contamination if the equipment is not properly cleaned between each use. |
Additional Online Courses
- What Every First Responding Officer Should Know About DNA Evidence
- Collecting DNA Evidence at Property Crime Scenes
- DNA – A Prosecutor’s Practice Notebook
- Crime Scene and DNA Basics
- Laboratory Safety Programs
- DNA Amplification
- Population Genetics and Statistics
- Non-STR DNA Markers: SNPs, Y-STRs, LCN and mtDNA
- Firearms Examiner Training
- Forensic DNA Education for Law Enforcement Decisionmakers
- What Every Investigator and Evidence Technician Should Know About DNA Evidence
- Principles of Forensic DNA for Officers of the Court
- Law 101: Legal Guide for the Forensic Expert
- Laboratory Orientation and Testing of Body Fluids and Tissues
- DNA Extraction and Quantitation
- STR Data Analysis and Interpretation
- Communication Skills, Report Writing, and Courtroom Testimony
- Español for Law Enforcement
- Amplified DNA Product Separation for Forensic Analysts