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Interlaboratory Comparison of SpermX and Conventional Differential Extractions

August 2022

Scientists from three participating laboratories - University of Central Florida (UCF), Center for Forensic Science Research and Education (CFSRE), and InnoGenomics (IGT) - presented results obtained from mock sexual assault samples using the SpermX method and their in-house differential extraction method. All three laboratories processed identical sets of female:male mixture swabs at various ratios in triplicate and presented their experiences and insights from this study.

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Pressure-based alkaline lysis with immunocapture a method for enhanced recovery in differential extraction

Journal

Electrophoresis

Date Published

2017

Agencies

NIJ-Sponsored

Confirmatory Detection of Sperm and Semen Via Proximity Ligation real-Time PCR

Journal

Forensic Science International Genetics Supplement Series

Date Published

December 2015

Agencies

NIJ-Sponsored

Raman Spectroscopic Method for Semen Identification: Azoospermia

Journal

Talanta

Date Published

March 2019

Agencies

NIJ-Sponsored

Automation of the Differential Digestion Process of Sexual Assault Evidence

Journal

Journal of Forensic Sciences

Date Published

2019

Agencies

NIJ-Sponsored

Proximity Ligation Real-Time PCR: A Protein-Based Confirmatory Method for the Identification of Semen and Sperm Cells From Sexual Assault Evidence

Journal

Forensic Science International-Genetics

Date Published

November 2018

Agencies

NIJ-Sponsored

Sperm Cell Purification From Mock Forensic Swabs Using SOMAmer (TM) Affinity Reagents

Journal

Forensic Science International-Genetics

Date Published

July 2018

Agencies

NIJ-Sponsored

Acoustic Differential Extraction for Forensic Analysis of Sexual Assault Evidence

Journal

Analytical Chemistry

Date Published

July 2009

Agencies

NIJ-Sponsored

Alternative direct-to-amplification sperm cell lysis techniques for sexual assault sample processing

Journal

Journal of Forensic Sciences

Date Published

2022

Agencies

NIJ-Sponsored

Evaluation and Verification of a microRNA Panel Using Quadratic Discriminant Analysis for the Classification of Human Body Fluids in DNA Extracts

Journal

Genes

Date Published

April 2023

Agencies

NIJ-Sponsored

Interlaboratory Comparison of SpermX and Conventional Differential Extractions

Event Dates

August 11, 2022, 1:00 pm – 2:00 pm Eastern

Location

Online

Event Type

Webinar

Registration Status

Not Required

Engineering the Interaction Dynamics between Nano-Topographical Immunocyte-Templated Micromotors across Scales from Ions to Cells

Journal

Small

Date Published

November 2020

Agencies

NIJ-Sponsored

Development and validation of a novel method "SpermX (TM)" for high throughput differential extraction processing of sexual assault kits (SAKs) for DNA analysis

Journal

Forensic Science International: Genetics

Date Published

July 2022

Agencies

NIJ-Sponsored

A Landscape Study Examining Technologies and Automation for Differential Extraction and Sperm Separation for Sexual Assault Investigations

Date Published

May 2022

Agencies

NIJ-Sponsored

Real-Time Detection and Control of Microchannel Resonance Frequency in Acoustic Trapping Systems by Monitoring Amplifier Supply Currents

Journal

Sensors

Date Published

2021

Agencies

NIJ-Sponsored

Interaction of age and temperature on heat shock protein expression, sperm count, and sperm viability of the adult black soldier fly (Diptera: Stratiomyidae)

Journal

Journal of Insects as Food and Feed

Date Published

2021

Agencies

NIJ-Sponsored

Bio-Inspired Magnetic Beads for Isolation of Sperm from Heterogenous Samples in Forensic Applications

Journal

Forensic Science International-Genetics

Date Published

2021

Agencies

NIJ-Sponsored

The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs

Journal

Analytical and Bioanalytical Chemistry

Date Published

2015

Agencies

NIJ-Sponsored

Development of DNA methylation markers for sperm, saliva and blood identification using pyrosequencing and qPCR/HRM

Journal

Analytical Biochemistry

Date Published

2020

Agencies

NIJ-Sponsored

Capillary zone electrophoresis separation and collection of spermatozoa for the forensic analysis of sexual assault evidence

Journal

Electrophoresis

Date Published

2020

Agencies

NIJ-Sponsored

A confirmatory test for sperm in sexual assault samples using a microfluidic-integrated cell phone imaging system

Journal

Forensic Science International-Genetics

Date Published

2020

Agencies

NIJ-Sponsored

Isolation of a Low Number of Sperm Cells from Female DNA in a Glass-PDMS-Glass Microchip via Bead-Assisted Acoustic Differential Extraction

Journal

Analytical Chemistry

Date Published

2019

Agencies

NIJ-Sponsored

Acoustic trapping of sperm cells from mock sexual assault samples

Journal

Forensic Sci Int Genet: Genetics Supplement Series

Date Published

2019

Agencies

NIJ-Sponsored

Real Time Electronic Feedback for Improved Acoustic Trapping of Micron-Scale Particles

Date Published

2019

Agencies

NIJ-Sponsored

Laser Microdissection Separation of Pure Spermatozoa From Epithelial Cells for Short Tandem Repeat Analysis

Journal

Journal of Forensic Sciences

Date Published

July 2006

Agencies

NIJ-Sponsored