Sperm detection and separation

Interlaboratory Comparison of SpermX and Conventional Differential Extractions

August 2022

Scientists from three participating laboratories - University of Central Florida (UCF), Center for Forensic Science Research and Education (CFSRE), and InnoGenomics (IGT) - presented results obtained from mock sexual assault samples using the SpermX method and their in-house differential extraction method. All three laboratories processed identical sets of female:male mixture swabs at various ratios in triplicate and presented their experiences and insights from this study.

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Pressure-based alkaline lysis with immunocapture a method for enhanced recovery in differential extraction

NCJ Number

256062

Journal

Electrophoresis

Date Published

2017

Agencies

NIJ-Sponsored

Publication Type

Research (Applied/Empirical)

HTML

Acoustic Differential Extraction for Forensic Analysis of Sexual Assault Evidence

NCJ Number

308350

Journal

Analytical Chemistry

Date Published

July 2009

Agencies

NIJ-Sponsored

Publication Type

Research (Applied/Empirical)

HTML

Evaluation and Verification of a microRNA Panel Using Quadratic Discriminant Analysis for the Classification of Human Body Fluids in DNA Extracts

NCJ Number

306856

Journal

Date Published

April 2023

Agencies

NIJ-Sponsored

Publication Type

Research (Applied/Empirical)

HTML

Interlaboratory Comparison of SpermX and Conventional Differential Extractions

Event Dates

August 11, 2022, 1:00 pm – 2:00 pm Eastern

Location

Online

Event Type

Webinar

Registration Status

Not Required

NCJ Number 304873 Thumbnail

A Landscape Study Examining Technologies and Automation for Differential Extraction and Sperm Separation for Sexual Assault Investigations

NCJ Number

304873

Date Published

May 2022

Agencies

NIJ-Sponsored

Publication Type

Best Practice/State-of-the-Art Review

HTML | PDF

Bio-Inspired Magnetic Beads for Isolation of Sperm from Heterogenous Samples in Forensic Applications

NCJ Number

304414

Journal

Forensic Science International-Genetics

Date Published

2021

Agencies

NIJ-Sponsored

Publication Type

Research (Applied/Empirical)

HTML

Laser Microdissection Separation of Pure Spermatozoa From Epithelial Cells for Short Tandem Repeat Analysis

NCJ Number

215137

Journal

Journal of Forensic Sciences

Date Published

July 2006

Agencies

NIJ-Sponsored

Publication Type

Report (Study/Research)

HTML

Towards Commercialization: Preliminary developmental validation of a high resolution melt curve mixture prediction assay and SVM tool

2019-DU-BX-0003

Past Project Period End Date

Funding First Awarded

2019

$399,699

Completion of the SONIC-DE 2.0 System for Implementation in Forensic Laboratories

2019-NE-BX-0004

Closed

Funding First Awarded

2019

$175,791

Bio-inspired Material-integrated Beads for Differential Extraction of Sperm in Forensic Applications

2019-NE-BX-0003

Closed

Funding First Awarded

2019

$499,545

Forensic DNA Research and Development

Emerging Forensic Research Series: Forensic Biology

Event Dates

April 9, 2019, 1:00 pm – 2:30 pm

Location

Online

Event Type

Webinar

Registration Status

Open

Better algorithms and chemistry for mixture interpretation

2018-DU-BX-0177

Closed

Funding First Awarded

2018

$694,525

Autosomal DNA-STR Profiling of Directly Captured Spermatozoa from Post-Coital (3-10 Days) Cervico-Vaginal Samples

2018-NE-BX-0001

Closed

Funding First Awarded

2018

$600,704

Resolving the Forensic DNA Mixture Problem through the Development of End-to-End Single-Cell Pipelines

2018-DU-BX-0185

Closed

Funding First Awarded

2018

$546,727

Body fluid analysis detection and identification by surface enhanced Raman spectroscopy for forensic scientists

2018-MU-MU-0013

Closed

Funding First Awarded

2018

$462,091

A Low-Cost Isothermal Amplification Microdevice for Rapid Colorimetric Detection Applied to Body Fluid Identification and Y-screening

2017-NE-BX-0008

Closed

Funding First Awarded

2017

$387,106

A Confirmatory Test for Sperm in Sexual Assault Samples using a Microfluidic-Integrated Cell Phone Imaging System

2017-NE-BX-0004

Closed

Funding First Awarded

2017

$369,224

Automation of a Sperm DNA Capture Assay for Forensic Applications

2016-DN-BX-0156

Closed

Funding First Awarded

2016

$499,994

Rapid Extraction of Sperm from Sexual Assault Kits

2016-DN-BX-0161

Closed

Funding First Awarded

2016

$209,408

Characterization and Quantification of Microbial Degradation of Sexual Lubricants

2016-DN-BX-0005

Closed

Funding First Awarded

2016

$50,000

Characterization of personal and condom lubricants using DART-TOFMS and comprehensive-GC-MS

2016-DN-BX-0004

Closed

Funding First Awarded

2016

$50,000

Highly efficient sperm cell separation from sexual assault samples for DNA analysis using novel polymer filter technology

2015-DN-BX-K072

Closed

Funding First Awarded

2015

$348,796

Enhancing Mixture Interpretation with Highly Informative STRs

2015-DN-BX-K067

Closed

Funding First Awarded

2015

$585,415