Proximity Ligation Real-Time PCR: A Protein-Based Confirmatory Method for the Identification of Semen and Sperm Cells From Sexual Assault Evidence

• NCJ Number: 254023
• Journal: Forensic Science International: Genetics Volume: 37 Dated: November 2018 Pages: 64-72
• Author(s): Sarah Riman; Chin H. Shek; Michéle A. Peck; Jaclyn Benjamin; Daniele Podini
• Date Published: November 2018
• Length: 9 pages
• Grant Number(s): 2012-DN-BX-K034
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical)
• Award: Proximity Ligation Real time PCR for the Detection of Spermatozoa

Annotation

This article reports on the use of the Proximity Ligation Real-Time PCR (PLiRT-PCR) assay as an attractive and promising confirmatory method for the identification of semen and sperm proteins, using two polyclonal antibodies, Prostate Specific Antigen (PSA) and Sperm-Specific Protein (SP10), respectively.

Abstract

The positive identification of seminal fluids in sexual assault crimes is considered crucial evidence to determine whether a sexual act occurred or not; however, current presumptive methods lack specificity and sensitivity. Confirmation of semen by microscopic examination of spermatozoa is laborious, time consuming, and can sometimes lead to negative or inconclusive results. In the procedures described in this article, PLiRT-PCR relies on protein recognition by pairs of proximity probes (antibody-DNA conjugates) that give rise to a ligated DNA strand. The ligated DNA strand is then amplified and detected by qPCR. (publisher abstract modified)