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Proximity Ligation Real-Time PCR: A Protein-Based Confirmatory Method for the Identification of Semen and Sperm Cells From Sexual Assault Evidence

NCJ Number
254023
Date Published
November 2018
Length
9 pages
Author(s)
Sarah Riman; Chin H. Shek; Michéle A. Peck; Jaclyn Benjamin; Daniele Podini
Agencies
NIJ-Sponsored
Publication Type
Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description
Grant Number(s)
2012-DN-BX-K034
Annotation
This article reports on the use of the Proximity Ligation Real-Time PCR (PLiRT-PCR) assay as an attractive and promising confirmatory method for the identification of semen and sperm proteins, using two polyclonal antibodies, Prostate Specific Antigen (PSA) and Sperm-Specific Protein (SP10), respectively.
Abstract
The positive identification of seminal fluids in sexual assault crimes is considered crucial evidence to determine whether a sexual act occurred or not; however, current presumptive methods lack specificity and sensitivity. Confirmation of semen by microscopic examination of spermatozoa is laborious, time consuming, and can sometimes lead to negative or inconclusive results. In the procedures described in this article, PLiRT-PCR relies on protein recognition by pairs of proximity probes (antibody-DNA conjugates) that give rise to a ligated DNA strand. The ligated DNA strand is then amplified and detected by qPCR. (publisher abstract modified)
Date Created: July 20, 2021