Body fluid analysis

The NIJ Forensic Technology Center of Excellence hosted a panel of subject matter experts to discuss emerging methods for body fluid analysis. This webinar is in three sections: 1) Proteomics and its potential in high-throughput forensic laboratories. 2) Surface Enhanced Raman Spectroscopy and the rapid detection and identification of body fluids. 3) MicroRNA, mRNA, and the development of a microfluidic device used to detect and differentiate body fluids from an unknown sample.

While forensic DNA analysis can identify an individual(s) at a crime scene, it cannot identify the biological source from which that DNA was obtained – e.g. blood, saliva, or semen. Identification of the biological source of DNA can provide important contextual information for forensic investigations as well as evidence in court. Nearly all current methods used for body fluid identification are presumptive. Here we present a new proteomic mass spectrometry method for the confirmatory identification of blood, saliva, and semen.

Scientists from three participating laboratories - University of Central Florida (UCF), Center for Forensic Science Research and Education (CFSRE), and InnoGenomics (IGT) - presented results obtained from mock sexual assault samples using the SpermX method and their in-house differential extraction method. All three laboratories processed identical sets of female:male mixture swabs at various ratios in triplicate and presented their experiences and insights from this study.