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Drug screening method development for paper spray coupled to a triple quadrupole mass spectrometer

NCJ Number
255254
Date Published
2017
Length
7 pages
Author(s)
Rachel Jett; Christine Skaggs; Nicholas E Manicke
Agencies
NIJ-Sponsored
Publication Type
Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description
Grant Number(s)
2014-R2-CX-K007
Annotation
This paper discusses method development for paper spray mass spectrometry on a triple quadrupole mass spectrometer, with attention to detection criteria, selected reaction monitoring (SRM) ratio tolerance, fragment ion selection, selectivity, and signal to blank considerations.
Abstract
Paper spray mass spectrometry is a direct analysis method in which compounds are extracted and ionized from biofluids dried on paper. It is an attractive option for performing rapid drug screening because it is fast (the entire sample analysis takes <2 minutes), simple (there is no sample preparation or chromatography), and sensitive (low ng mL 1 levels of many drugs can be detected from dried blood spots). In the current study, 134 drugs and drug metabolites commonly encountered in forensic toxicological analyses were spiked into blood at concentrations reasonable for post-mortem drug screening applications. Detection of some 130 drug and drug metabolites using a single paper spray MS/MS was shown for these spike and recover experiments. (publisher abstract modified)
Date Created: July 20, 2021