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A Capillary Electrophoresis Method for Identifying Forensically Relevant Body Fluids Using miRNAs

NCJ Number
Legal Medicine Volume: 30 Dated: January 2018 Pages: 1-4
Date Published
January 2018
4 pages
This study designed a preliminary 8-marker system for body fluid identification (BFID) that includes an endogenous reference gene (let-7g) to differentiate between venous blood (miR-451a and miR-142-3p), menstrual blood (miR-141-3p and miR-412-3p), semen (miR-891a and miR-10b), and saliva (miR-205), using a capillary electrophoresis approach.

Body fluid identification (BFID) can provide crucial information during an investigation. In recent years, microRNAs (miRNAs) have shown considerable body fluid specificity, are able to be co-extracted with DNA, and their small size (18-25 nucleotides) makes them ideal for analyzing highly degraded forensic samples. The panel used in the current study is a linear primer system, in order to incorporate additional miRNA markers by forming a multiplex system. The miRNA system was able to distinguish between venous blood, menstrual blood, semen, and saliva, using a rudimentary data interpretation strategy. All STR amplifications from co-extracted DNA yielded complete profiles for human identification purposes. (publisher abstract modified)

Date Published: January 1, 2018