This study examined next-generation sequencing (NGS) analyte signal variation, specifically heterozygous balance and stutter variability from profiles generated using the ForenSeq™ DNA Signature Prep Kit, DNA Primer Mix B.
There has been an increase in the number of laboratories and researchers adopting new sequencing technologies, known as next-generation sequencing (NGS). An understanding of the behavior of NGS DNA profiles is needed to enable the development of probabilistic genotyping methods for the interpretation of such profiles. The current study also investigated additivity of analyte signals in NGS profiles for overlapping allelic and stutter signals originating from the same or different contributors. Models are described that can be used to inform a continuous method for the interpretation of DNA profiling data. (publisher abstract modified)