This article presents a novel method to determine time since deposition of trace DNA samples presented in forensic cases to answer questions about how and when the DNA was deposited.
With the increase in sensitivity of DNA profiling, questions about how and when the DNA was deposited have become a driving issue in forensic cases. To address this, the authors propose a novel method to determine time since deposition of trace DNA samples based on morphological and autofluorescence properties of individual epithelial cells which can change as the sample ages. To develop this signature, a series of trace DNA samples were generated by contact/handling a substrate and then allowed to age anywhere between one day and more than one year prior to collection. Imaging flow cytometry (IFC) was then used to characterize the morphology and autofluorescence profiles of individual cells within each sample followed by multivariate modelling and predictive classification. (Published Abstract Provided)
Downloads
Similar Publications
- Factors Affecting Species Identifications of Blow Fly Pupae Based upon Chemical Profiles and Multivariate Statistics
- A Systematic Study of Liquid Chromatography to Separate Eighteen Natural Cannabinoids for Potency Testing of Hemp-Based Products Using Diode Array Detector and Electrospray Ionization Mass Spectrometry
- Forensic transcriptome analysis using massively parallel sequencing