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Volume Reduction Solid Phase Extraction of DNA From Dilute, Large-Volume Biological Samples

NCJ Number
230529
Date Published
Author(s)
Naila Bhatri, Joan M. Bienvenue, Lisa Coletta, Susan Greenspoon, James P. Landers, Carmen R. Reedy, Briony C. Strachan
Annotation
SPE was carried out on a microdevice utilizing magnetic silica particles and an optimized volumetric flow rate and elution buffer, resulting in a 50-fold decrease in volume and a 15-fold increase in DNA concentration.
Abstract
Microdevices are often designed to process sample volumes on the order of tens of microliters and cannot typically accommodate larger volume samples without adversely affecting efficiency and greatly increasing analysis time. However, dilute, large-volume biological samples are frequently encountered, especially in forensic or clinical laboratories. A microdevice, capable of efficiently processing 0.5-1mL samples has been developed for solid phase extraction (SPE) of DNA. Device characterization studies showed DNA extraction efficiencies comparable with previously reported silica-based purification methods, with robust performance demonstrated by the successful amplification of a fragment from the gelsolin gene extracted from dilute whole blood. In addition, the microchip-based method for SPE of large volume, dilute samples was also used to demonstrate the first successful on-chip purification of mitochondrial DNA (mtDNA) from both dilute whole blood and a degraded blood stain. Figures and references (Published Abstract)
Date Created: March 31, 2010