Working Towards Implementation of Whole Genome Mitochondrial DNA Sequencing into Routine Casework

• NCJ Number: 253162
• Journal: Forensic Science International: Genetics Volume: 6 Dated: December 2007 Pages: E-388-E-389
• Author(s): Jennifer D. Churchill; Dixie Peters; Christina Capt; Christina Strobl; Walther Parson; Bruce Budowle
• Date Published: December 2007
• Length: 2 pages
• Grant Number(s): 2016-DN-BX-K001
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical); Case Study
• Award: Evaluation and Implementation of High Throughput Second Generation Sequencing for Mitochondrial DNA Testing in Missing Persons and Forensic Casework at the UNT Center for Human Identification.

Annotation

The authors describe how their lab is working toward the implementation of whole genome mitochondrial DNA sequencing into routine casework.

Abstract

Massively parallel sequencing (MPS) technologies offer opportunities to expand forensic mitochondrial DNA (mtDNA) typing capabilities, affording higher resolution, an increase in discrimination power, and potentially enabling mixture interpretation. In addition, improvements to MPS technologies and workflows have made implementation into forensic genetic laboratories increasingly more feasible. Following initial successful studies with the Precision ID mtDNA Whole Genome Panel, the process of internal validation and implementation of whole genome mtDNA sequencing began in the authors Missing Persons and Forensic Casework Units. (publisher abstract modified)