Understanding Metal Inhibition: The Effect of Copper (Cu2+) on DNA Containing Samples

• NCJ Number: 252341
• Journal: Forensic Chemistry Volume: 4 Dated: June 2017 Pages: 89-95
• Author(s): Liliana I. Moreno; Bruce R. McCord
• Date Published: June 2017
• Length: 7 pages
• Grant Number(s): 2010-DN-BX-K204
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical); Report (Study/Research); Report (Grant Sponsored); Program/Project Description
• Award: Detection and remediation of PCR inhibition using real time PCR melt curves as a diagnostic tool.

Annotation

This project examined whether copper ions in an evidence sample that contains DNA have adverse effects on DNA processing.

Abstract

Copper wires are a common component of improvised explosive devices (IEDs) and are increasingly found in forensic evidence. When processed for DNA, ions from the metal can be co-extracted with the genetic material, and potentially cause complications during the analysis procedure. It was determined in this project that copper ions do have adverse effects on DNA processing by either interacting directly with the DNA or affecting the electrophoretic mobility of the molecule. It was also found that these negative effects could be improved by using a purification method capable of reducing the amount of copper carry-over into the sample. (Publisher abstract modified)