Description of original award (Fiscal Year 2010, $294,800)
This project builds on previously NIJ funded work to examine and characterize mechanisms for degradation and inhibition of DNA samples. The goal of this proposal to examine the capability of a commercially available real-time PCR system (Plexor HY) to assess and identify classes of inhibitors a priori through the use of melt curve effects. Researches propose to look at ways to enhance the capability of Plexor to detect inhibition by modifying the chemistry of the reaction mixture. Based on data generated from previous work, researchers also propose to develop better internal positive control sequence (IPC) probes that are more efficient at detecting inhibitors that bind DNA. Using this data researchers will categorize various inhibitors based on their effect on amplification of Identifiler and Powerplex 16, and ultimate plan to assess various methods to relieve inhibition including sample dilution, spin filtration, and magnetic bead based extraction.
- Implementation of NPS Discovery – An Early Warning Systems for Novel Drug Intelligence, Surveillance, Monitoring, Response, and Forecasting using Drug Materials and Toxicology Populations in the US
- Measuring rarity of combined features in palm versus finger impressions and evaluating examiner perceptions
- Touch DNA in Activity-Level Propositions: A Bullpen of Events to Inform Alternate Prosecution and Defense Scenarios