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SNP-microarrays Can Accurately Identify the Presence of an Individual in Complex Forensic DNA Mixtures

NCJ Number
249336
Author(s)
Lev Voskoboinik, Sheri B. Ayers, Aaron K. LeFebvre, Ariel Darvasi
Date Published
May 2015
Length
8 pages
Annotation
This study evaluated the practical usefulness of currently available “off-the-shelf” SNP microarrays for identifying the presence of an individual in a mixture of several contributors in DNA samples from a mass disaster.
Abstract
The study found that a set of 3000 SNPs specifically selected for this purpose can accurately identify the presence of an individual in complex DNA mixtures of various compositions; for example, individuals contributing as little as 5 percent to a complex DNA mixture can be robustly identified even when the starting DNA amount is as little as 5.0 ng and had undergone whole-genome amplification (WGA) prior to SNP analysis. The work presented in this study represents proof-of-principle that the authors previously proposed approach can work with real “forensic-type” samples. Furthermore, in the absence of a low-density focused forensic SNP microarray, the use of standard, currently available high-density SNP microarrays can be similarly used and even increase statistical power due to the larger amount of available information. (Publisher abstract modified)
Date Published: May 1, 2015