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Forensic Identification of an Individual in Complex Mixtures Utilizing SNP Technology

Award Information

Awardee
Award #
2011-DN-BX-K555
Location
Congressional District
Status
Closed
Funding First Awarded
2011
Total funding (to date)
$224,968

Description of original award (Fiscal Year 2011, $224,968)

This research will develop a method using a SNP assay based on the recent work elucidating a novel strategy for identification of an individual's DNA in a complex mixture composed of up to 10 individuals. This strategy employs genotyping a DNA mixture and a 'suspect' DNA sample with 1000-3000 SNPs. The statistical framework shows that this number of markers produces the statistical power required for accurate assessment and inclusion/exclusion of a single contributor in a complex mixture. The research aims to develop and thoroughly test the feasibility of this strategy utilizing Illumina's GoldenGate(R) genotyping technology, a SNP genotyping technology commonly utilized by the forensic community. Researchers will test and optimize a pre-selected SNP panel. Following optimization of the SNP panel, thorough testing of detection limits, sensitivity, and reproducibility studies will be performed prior to testing complex mixtures containing between 3-10 individuals. ca/ncf

Date Created: September 11, 2011