Forensic Identification of an Individual in Complex Mixtures Utilizing SNP Technology

This research will develop a method using a SNP assay based on the recent work elucidating a novel strategy for identification of an individual's DNA in a complex mixture composed of up to 10 individuals. This strategy employs genotyping a DNA mixture and a 'suspect' DNA sample with 1000-3000 SNPs. The statistical framework shows that this number of markers produces the statistical power required for accurate assessment and inclusion/exclusion of a single contributor in a complex mixture. The research aims to develop and thoroughly test the feasibility of this strategy utilizing Illumina's GoldenGate(R) genotyping technology, a SNP genotyping technology commonly utilized by the forensic community. Researchers will test and optimize a pre-selected SNP panel. Following optimization of the SNP panel, thorough testing of detection limits, sensitivity, and reproducibility studies will be performed prior to testing complex mixtures containing between 3-10 individuals. ca/ncf