This study introduces a rotationally-driven dynamic solid phase sodium bisulfite conversion disc for forensic epigenetic sample preparation.
Epigenetic analysis requires sodium bisulfite conversion (BSC), a sample preparation method that is time-consuming, labor-intensive, prone to contamination, and characterized by DNA loss and fragmentation. To provide an alternative method for BSC that is more amenable to integration with the forensic DNA workflow, the authors of this study describe a rotationally-driven, microfluidic method for dynamic solid phase-BSC (dSP-BSC) that streamlines the sample preparation process in an automated format, capable of preparing up to four samples in parallel. The method permitted decreased incubation intervals by ∼36% and was assessed for relative DNA recovery and conversion efficiency and compared to gold-standard and enzymatic approaches. (Published Abstract Provided)
Downloads
Similar Publications
- Assessing Methods to Enhance and Preserve Proteinaceous Impressions from the Skin of Decedents during the Early Stages of Decomposition
- A Black Box Study of the Accuracy and Reproducibility of Tire Evidence Examiners’ Conclusions
- A Reflective Spectroscopy and Mineralogical Investigation of Cosmetic Blush (Wet‘N’Wild) Potentially for Forensic Investigations Related to Interpersonal Violence—An Experimental Feasibility Study