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Raman Spectroscopy of Blood for Species Identification

NCJ Number
Gregory McLaughlin, Kyle C. Doty, Igor K. Ladnev
Date Published
December 2014
6 pages
This article reports a satisfactory classification of blood obtained from 11 animal classes and human subjects by statistical analysis of Raman spectra.
The classification of blood samples was achieved according to each sample’s species of origin, which enhanced previously observed discrimination ability. The developed approach does not require the knowledge of a specific (bio)chemical marker for each individual class but rather relies on a spectroscopic statistical differentiation of various components. This approach results in remarkable classification ability even with intrinsically heterogeneous classes and samples. In addition, the obtained spectroscopic characteristics could potentially provide information about specific changes in the (bio)chemical composition of samples, which are responsible for the differentiation. The species identification of a blood stain is an important and immediate challenge for forensic science, veterinary purposes, and wildlife preservation. The current methods used to identify the species of origin of a blood stain are limited in scope and destructive to the sample. The authors previously demonstrated that Raman spectroscopy can reliably differentiate blood traces of human, cat, and dog (Virkler et al. Anal. Chem. 2009, 81, 7773-7777) and, most recently, built a binary model for differentiating human vs animal blood for 11 species integrated with human existence (McLaughlin et al. Forensic Sci. Int. 2014, 238, 91-95). (Publisher abstract modified)
Date Published: December 1, 2014