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Quantitative Analysis of Desomorphine in Blood and Urine Using Solid Phase Extraction and Gas Chromatography-Mass Spectrometry

NCJ Number
254105
Date Published
February 2019
Length
7 pages
Author(s)
Jessica Winborn; Sarah Kerrigan
Agencies
NIJ-Sponsored
Annotation
Since the lack of confirmed desomorphine reports may be in part due to the limited published analytical methodology capable of detecting desomorphine at forensically relevant concentrations, the goal of this study was to assist with identification efforts by developing and validating a robust analytical method.
Abstract
Desomorphine, a semi-synthetic opioid, is a component of the street drug Krokodil. Despite continued reports of Krokodil use, confirmation via toxicological testing remains scarce. In the current project, Solid phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS) were used to determine desomorphine in blood and urine, using a deuterated analog as the internal standard. Data were acquired using selected ion monitoring (SIM) mode. Extraction efficiencies in blood and urine were 69 percent and 90 percent, respectively. The limits of quantitation in blood and urine were 5 ng/mL and 8 ng/mL, ten-fold lower than previously published methods. Intra- and inter-assay CVs were 2-4 percent (n=3) and 3-7 percent (n 15), respectively. The method was fully validated in accordance with published guidelines for forensic use, and it provides a means for identifying desomorphine in toxicology specimens at forensically relevant concentrations, without the need for derivatization. (publisher abstract modified)

Date Created: July 20, 2021