This study reviewed new approaches for the streamlined development of high-performance aptamer-based sensors for small-molecule detection.
Aptamers are nucleic acid-based affinity reagents that have gained widespread attention as biorecognition elements for the detection of targets such as ions, small molecules, and proteins. Over the past three decades, the field of aptamer-based sensing has grown considerably; however, the advancement of aptamer-based small-molecule detection has fallen short of the high demand for such sensors in applications such as diagnostics, environmental monitoring, and forensics. This is due to two challenges: the complexity of developing generalized sensing platforms and the poor sensitivities of assays targeting small molecules. The current study provides historical context, explores the current state-of-the art, and offers future directions—with emphasis on new aptamer engineering methods, the use of cooperative binding, and label-free approaches using fully-folded, high-affinity aptamers for small-molecule sensing. (publisher abstract modified)
Downloads
Similar Publications
- The Efficacy of County-Level Sex Offender Residence Restrictions in New York
- A novel loop-mediated isothermal amplification method for identification of four body fluids with smartphone detection
- A memory-efficient algorithm to obtain splicing graphs and de novo expression estimates from de Bruijn graphs of RNA-Seq data