NCJ Number
231046
Date Published
May 2010
Length
2 pages
Annotation
This paper describes the innovative denaturing high-performance liquid chromatography (DHPLC) method for profiling DNA.
Abstract
After years of research, the denaturing high-performance liquid chromatography (DHPLC) originally developed as a biomedical technology for medical applications was adapted to forensic application. DHPLC allows for the rapid and accurate separation of mixtures that contain the mitochondrial DNA (mtDNA) of more than one person. The DHPLC method has the potential to benefit criminal and mass disaster investigations requiring timely analysis of mtDNA. This paper describes two major forensic applications for DHPLC in expediting mtDNA processing. The first application is comparative sequence analysis, and the second application of DHPLC application is the ability to separate mixtures that contain the mtDNA of more than one person.
Date Published: May 1, 2010
Downloads
Similar Publications
- Extraction of Ignitable Liquid Residues by Dynamic Capillary Headspace Sampling and Comparison to the Carbon Strip Method
- Development and Evaluation of a Nontargeted Electrochemical Surface-Enhanced Raman Spectroscopy (EC-SERS) Screening Method Applied to Authentic Forensic Seized Drug Casework Samples
- Development and Validation of a Method for Analysis of 25 Cannabinoids in Oral Fluid and Exhaled Breath Condensate