U.S. flag

An official website of the United States government, Department of Justice.

Discriminant Analysis of Raman Spectra for Body Fluid Identification for Forensic Purposes

NCJ Number
Sensors Volume: 10 Issue: 4 Dated: 2010 Pages: 2869-2884
Date Published
16 pages

Since the detection and identification of blood, semen and saliva stains, the most common body fluids encountered at a crime scene, are important aspects of forensic science, the current study aimed to develop a nondestructive, confirmatory method for body-fluid identification based on Raman spectroscopy coupled with advanced statistical analysis.


Dry traces of blood, semen and saliva obtained from multiple donors were probed using a confocal Raman microscope with a 785-nm excitation wavelength under controlled laboratory conditions. Results demonstrated, with high confidence, the capability of Raman spectroscopy to identify an unknown substance to be semen, blood or saliva. 5 figures, 1 table, and 75 references (publisher abstract modified)

Date Published: January 1, 2010