Since the United States and many other countries worldwide are currently experiencing a public health crisis due to the abuse of heroin and illicitly manufactured fentanyl, the current project developed a liquid chromatography tandem mass spectrometry (LC–MS-MS)-based method for the detection of morphine, fentanyl, and their metabolites, including morphine-3-glucuronide (M3G), morphine-6-glucuronide (M6G), normorphine, norfentanyl and deuterated internal standards in limited sample volumes with the limit of detection of 5.0/0.5 ng/mL (morphine, M3G, M6G, normorphine/fentanyl, norfentanyl).
The inter-assay precision (percent CV) was less than 12 percent for all assays, and the inter-assay bias (percentage) was less than 5 percent. The ruggedness of the method, dilution effect, and carryover were also investigated as part of the study. The simultaneous quantification of morphine, fentanyl, and its metabolites with this simple and time- and cost-efficient method could be successfully applied to samples taken for pharmacokinetic evaluation (antemortem and postmortem) after a single dose of morphine or co-administration of morphine with other drugs (e.g., fentanyl) in rats. (publisher abstract modified)
Downloads
Similar Publications
- Linking Ammonium Nitrate – Aluminum (AN-AL) Post-Blast Residues to PreBlast Explosive Materials Using Isotope Ratio and Trace Elemental Analysis for Source Attribution
- Development of a Liquid Chromatographic Method with a Different Selectivity for the Quantification of Eighteen Phytocannabinoids in Hemp
- New Screening Method to Detect Drugs and Poisons Postmortem