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A Capillary Eelectrophoresis Method for Identifying Forensically Relevant Body Fluids Using miRNAs

NCJ Number
253325
Date Published
January 2018
Length
4 pages
Author(s)
Carrie Mayes; Sarah Seashots-Williams; Sheree Hughes-Stamm
Agencies
NIJ-Sponsored
Publication Type
Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description
Grant Number(s)
2016-DN-BX-0001
Annotation
This study designed a preliminary 8-marker system for body fluid identification (BFID), including an endogenous reference gene (let-7g), to differentiate between venous blood (miR-451a and miR-142-3p), menstrual blood (miR-141-3p and miR-412-3p), semen (miR-891a and miR-10b), and saliva (miR-205) using a capillary electrophoresis approach.
Abstract
Body fluid identification (BFID) can provide crucial information during an investigation. In recent years, microRNAs (miRNAs) have shown considerable body fluid specificity, are able to be co-extracted with DNA, and their small size (18-25 nucleotides) makes them ideal for analyzing highly degraded forensic samples. The panel of the current study used a linear primer system in order to incorporate additional miRNA markers by forming a multiplex system. The miRNA system was able to distinguish between venous blood, menstrual blood, semen, and saliva using a rudimentary data interpretation strategy. All STR amplifications from co-extracted DNA yielded complete profiles for human identification purposes. (publisher abstract modified)
Date Created: July 20, 2021