Applications of epigenetic methylation in body fluid identification, age determination and phenotyping

• NCJ Number: 302740
• Journal: Forensic Science International Genetics Supplement Series Volume: 7 Issue: 1 Dated: 2019 Pages: 485-487
• Author(s): B. McCord; et al
• Date Published: 2019
• Length: 3 pages
• Grant Number(s): 2017-NE-BX-0001
• Agencies: NIJ-Sponsored
• Award: Implementation of epigenetics into forensic science: Development of a PCR based multiplex for the simultaneous analysis of age and body fluid identification

Annotation

In this paper, four different applications are presented in forensic epigenetics using bisulfite modified PCR, HRM, and pyrosequencing. 

Abstract

The first application involves the development of a multiplex amplification capable of simultaneous analysis of four different body fluid/cell types- blood, saliva, sperm, and vaginal epithelia. The authors have also developed a method for the detection of sperm high-resolution melt analysis (HRM). Two other applications involve a determination of suspect age and a biomarker for determining smoking status. (publisher abstract modified)