Description of original award (Fiscal Year 2007, $324,705)
Biological samples collected from sexual assault or homicide victims can contain very low levels of male DNA among a large number of female epithelial cells. This often results in failure to obtain a traditional STR typing from the male DNA donor. Y chromosome specific genetic markers have become invaluable for the identification of the genetic profile of the male component in mixed male/female specimens. However, despite the presence in a stain of potentially probative biological evidence, a number of factors may preclude the ability to obtain a Y STR profile from the donor. The purpose of this academic-industry partnership project is to use a 'whole genome amplification' method to selectively enrich for Y chromosomal DNA prior to Y chromosome genetic marker analysis.
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