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Effects of DNA Extraction Methods on Recovery, Degradation, and Loss

Event Dates
Eastern
Event Duration
1 hour
Location
Online

DNA recovered from forensic and ancient DNA (aDNA) sources is generally expected to be in low copy number and degraded in strand length. However, considering the following equation, it is important to realize that the exact amount of recoverable DNA from any source is unknown:

Net yield of DNA= Original amount – loss in sampling – loss in extraction/purification – loss due to amplification bias (e.g., due to PCR inhibitors)

This study’s first objectives include estimating: 1) the amount of DNA lost during DNA extraction and purification, and 2) the degree to which molecules are damaged as a result of the extraction method.

Learning objectives:

  1. To illustrate that we do not know how much DNA is available from any given sources. Analysts can only know how much is retained through the process of its extraction and purification. With regards to highly compromised DNA sources, there may exist more DNA than is currently recognized.
  2. To illustrate the influence of various DNA extraction methods on the quantity (i.e., concentration) and quality (i.e., strand length) of resulting DNA eluates.
  3. To provide a better illustration of the nature of DNA loss from degraded and low concentration sources when utilizing commonly used silica-based extraction methods.

Event Type
Webinar
Event Option(s)
Online, On demand
Sponsor(s) / Host(s)
National Institute of Justice
Contact
NIJ Forensic Technology Center of Excellence
Registration Dates
August 11, 2020 - August 20, 2020
Registration Status
Open
Eligibility
Free and open to all
Credits

Certificate of completion

Date Modified:

 

    Date Created: August 12, 2020