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Many working with genomic DNA can afford to lose large portions of it during its extraction without negatively impacting downstream analysis. However, with work targeting highly degraded DNA sources, retention of every molecule can be key to successful analyses. This webinar addressed the insufficient documentation of absolute efficiencies of extraction methods and the nature of DNA loss associated with the study of low copy number and degraded DNA samples.
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This webinar highlighted the results of a full developmental validation of a method that estimates the A Posteriori Probability (APP) of the number of contributors in a DNA sample(NOC), and demonstrated that estimating the APP based on peak heights outperforms methods that rely on binary determinations alone. This presentation focused on including the true NOC in Likelihood Ratio interpretations and on repeatability.
This webinar was presented by the NIJ Forensic Technology Center of Excellence on July 14, 2020.
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