NCJ Number
252406
Date Published
June 2017
Length
7 pages
Annotation
This article reports on a research project that investigated sonication as a primary means of non-destructive DNA extraction from carrion flies.
Abstract
Since entomological evidence can be critical in establishing a postmortem interval estimate, DNA-based species identification can be an extremely valuable tool for forensic entomology. The current research examined whether sonication can process such samples in a consistent, cost-effective manner that retains the morphological attributes of the specimen for vouchering. Researchers analyzed the efficacy of this technique and compared it to an established DNA extraction technique — the Qiagen DNeasy tissue kit. The results indicate that sonication produces a significant reduction in the sequence length and lower PHRED quality scores when compared to sequences using DNA obtained using the DNeasy kit, but species identification and phylogenetic inferences between sonication and DNeasy extractions are equivalent. (Publisher abstract modified)
Date Published: June 1, 2017
Downloads
Similar Publications
- Recommendations for the forensic analysis and interpretation of glass from contemporary portable electronic devices by refractive index measurement and micro-X-ray fluorescence spectrometry
- Predicting thermal response of gypsum board under various heat flux Configurations: A three-dimensional mathematical model
- Development of baseline survey of random presence of glass and paint for the interpretation of evidence in the US courts