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QuantAssure Cassette Development Research

NCJ Number
Date Published
43 pages
This research examines the development of a lateral flow cassette to identify the presence of sperm and semen in evidence samples.
Results conclude that the effort to develop a polyclonal antibody by immunizing rabbits with the SPTRX3 exon5 peptide failed. The inability to develop SPTRX3 antibodies during the course of this project has prevented the intended effect of making the processing of rape kits in the forensic laboratory faster and more effective. BluePoint Bioscience immunized mice with the SPTRX3 exon1 peptide in order to develop a monoclonal antibody. This effort at SPTRX3 antibody development also failed. A second round of mouse immunizations met with the same fate. The inability to develop SPTRX3 antibodies brought the research project to a close. However, it is believed that the concept of developing a lateral flow cassette to determine the presence of spermatozoa and semen in evidence samples remains viable. Tables, figures, and references

Date Published: January 1, 2011