Investigators who use next generation DNA sequences to identify individuals may encounter degraded or mixed samples. Using funding from the National Institute of Justice (NIJ), Cassandra Calloway, a scientist at Children’s Hospital Oakland Research Institute (California), has developed a technique to make fragmented DNA more discernible. She also designed, tested, and implemented an analytic tool that generates individual DNA profiles from a variety of mixture proportions. Both of these methods can be used for DNA profiling of samples too aged or damaged, such as those from the 9/11 attacks or the recent California wildfires, to produce any identifying information using standard forensic short tandem repeats. This article describes Calloway’s research.