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Modified DOP-PCR for Improved STR Typing of Degraded DNA From Human Skeletal Remains and Bloodstains

NCJ Number
250028
Author(s)
A. Ambers, M. Turnbough
Date Published
January 2016
Length
6 pages
Annotation
Several strategies have been proposed to address the challenges posed by degraded and low copy templates, including a PCR-based whole genome amplification method called degenerate oligonucleotide-primed PCR (DOP-PCR). This study assessed the efficacy of four modified versions of the original DOP-PCR primer that retain at least a portion of the 5Œ defined sequence and alter the number of bases on the 3Œ end.
Abstract
The use of each of the four modified primers resulted in improved STR profiles from environmentally-damaged bloodstains, contemporary human skeletal remains, American Civil War era bone samples, and skeletal remains of WWII soldiers over those obtained by previously described DOP-PCR methods and routine STR typing. Additionally, the modified DOP-PCR procedure allows for a larger volume of DNA extract to be used, reducing the need to concentrate the sample and thus mitigating the effects of concurrent concentration of inhibitors. (Publisher abstract modified)
Date Published: January 1, 2016