Mitochondrial and STR DNA Analysis by Mass Spectrometry Using the Ibis Biosciences, Inc. Platform - A Technology Transition Workshop

• NCJ Number: 236452
• Author(s): National Forensic Science Technology Center
• Date Published: February 2009
• Length: 5 pages
• Agencies: NIJ-Sponsored

Annotation

The purpose of this Technology Transition Workshop is to provide the student with an introduction to biological mass spectrometry; the Ibis T5000™ and T6000™ platforms, their applications, and assay protocols; an overview of the T5000™ hardware and its maintenance; and the use of the Ibis software for data analysis.

Abstract

The purpose of this Technology Transition Workshop is to provide the student with an introduction to biological mass spectrometry; the Ibis T5000™ and T6000™ platforms, their applications, and assay protocols; an overview of the T5000™ hardware and its maintenance; and the use of the Ibis software for data analysis. Students will also receive instruction on mitochondrial DNA analysis; mitochondrial DNA mixture detection, analysis, and interpretation; statistics of polymorphic STR alleles; and future directions of biological mass spectrometry. Use of the Ibis T5000™ Mass Spectrometer Platform will be demonstrated, allowing students to take samples that they amplify through analysis and data interpretation using Ibis mtDNA software.