We describe a procedure to address calculation of match probabilities when casework samples are generated using an MPS kit with different trim sites than those present in the relevant population frequency database.
The first autosomal sequence-based allele (aka SNP-STR haplotype) frequency database for forensic massively parallel sequencing (MPS) has been published, thereby removing one of the remaining barriers to implementing MPS in casework. The database was developed using a specific set of flank trim sites. If different trim sites or different kits with different primers are used for casework, then SNP-STR haplotypes may be detected that do not have frequencies in the database. The proposed procedure provides a framework for comparison of any MPS kit or database combination while also accommodating comparison of MPS and CE profiles. (Publisher abstract provided)
Downloads
Similar Publications
- Development and Validation of a Method for Analysis of 25 Cannabinoids in Oral Fluid and Exhaled Breath Condensate
- Recovery and Detection of Ignitable Liquid Residues from the Substrates by Solid Phase Microextraction – Direct Analysis in Real Time Mass Spectrometry
- Direct Comparison of Body Fluid Identification Technologies